Apparatus and methods for collecting stem cells from an umbilical cord utilize a plurality of needles that are inserted into the umbilical cord. The needles may be fenestrated, and may have a common output to a line via which the stem cells are collected. A negative pressure source, such as a vacuum, may be used to draw the cells through the needles and the line. Apparatus for holding the umbilical cord, compressing the umbilical cord, positioning the plurality of needles in relation to the umbilical cord, guiding insertion of the needles toward and into the umbilical cord, withdrawing blood from the umbilical cord, and/or collecting blood withdrawn from the umbilical cord may be provided. One or more one-way check valves may be used to control fluid flow. Additionally or alternatively, a shunt may be used during blood collection to prevent backflow through the needles.
