PROBLEM TO BE SOLVED: To provide a culturing method which makes it possible to stably secure aphanothece sacrum.SOLUTION: This invention provides a culturing method where aphanothece sacrum purified through a crushing step that crushes aphanothece sacrum with a homogenizer, a filtration step that filtrates the aphanothece sacrum after the crushing step to clean it with water, and an ultrasound application step that applies ultrasound to the aphanothece sacrum after the cleaning step to clean it with water is cultured in a culture medium comprising ions of Na, K, Mg, Ca, N, P, S, and Cl.COPYRIGHT: (C)2015,JPO&INPIT【課題】スイゼンジノリの安定的確保を可能にする培養方法を提供する。【解決手段】スイゼンジノリをホモジナイザーで破砕する破砕工程と,破砕工程後のスイゼンジノリをろ過して水で洗浄するろ過工程と,洗浄工程後のスイゼンジノリに超音波を照射し水で洗浄する超音波照射工程により精製したスイゼンジノリを、Na,K,Mg,Ca,N,P,S,Clからなる各イオンを含む培養液で培養する培養方法。【選択図】図4