The animal “I” maze is a novel maze to assess anxiety behavior in mice. The “I” maze comprise of a white straight platform of 48 cm length and 5 cm width. Maze is divided equally into three arms; one central open arm, surrounded on outer edges by 6 mm high “lip”. This open arm is in continuation with two terminal closed arms that are positioned such that one closed arm is at each side of open arm. Each closed arm has a 6 cm high wall. The fabrication material may be wooden or any other temperature buffering material that may be helpful to resist change in temperature of the surface of the maze platform.