The present invention provides a medium for stem cell proliferation comprising a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration promoting the growth of a stem cell in the presence of FGF, a culture method of a stem cell using a medium comprising FGF for stem cell proliferation, comprising adding a sulfated compound or a pharmaceutically acceptable salt thereof to the medium at a concentration promoting the growth of a stem cell in the presence of FGF and the like.