Described herein are methods of assessing a wide variety of physiological needs a subject (e.g., a human patient) may have as a result of an internally-driven or externally-imposed event. Aspects of the methods are computer-aided and can be used to assess a subject's need for nutritional or medicinal support. Accordingly, the invention features computer systems configured to carry out the methods described herein and computer-readable media containing program code for performing the methods. The invention also encompasses the generation of biological translation curves, and the information obtained by the present methods can be extended to include therapeutic methods that rely on complex analyses of a plurality of analytes related to a given biomarker. The invention also features pharmaceutical or physiologically acceptable compositions that are tailor-made for a given subject (e.g., a human patient) or group of subjects (e.g., a herd of livestock or crop of plants).
