COOL, SIMON MCKENZIE,NURCOMBE, VICTOR,DOMBROWSKI, CHRISTIAN
申请号：
NZ59166809
公开号：
NZ591668A
申请日:
2009.02.19
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Disclosed is a method of identifying glycosaminoglycans capable of stimulating or inhibiting the growth and/or differentiation of cells and/or tissues, the method comprising: (i) providing a solid support having polypeptide molecules adhered to the support, wherein the polypeptide comprises a heparin-binding domain； (ii) contacting the polypeptide molecules with a mixture comprising glycosaminoglycans such that polypeptide-glycosaminoglycan complexes are allowed to form； (iii) partitioning polypeptide-glycosaminoglycan complexes from the remainder of the mixture； (iv) dissociating glycosaminoglycans from the polypeptide-glycosaminoglycan complexes； (v) collecting the dissociated glycosaminoglycans； (vi) adding the collected glycosaminoglycans to cells or tissues in which a protein containing the amino acid sequence of the heparin-binding domain is present； (vii) measuring one or more of: proliferation of the cells, differentiation of the cells, expression of one or more protein markers.
