The invention discloses a method for renaturation and purification of Recombinant Human Granulocyte Colony-stimulating Factor. In particular, the invention removes the reducing agent in the form of buffer replacement prior to renaturation, thereby improving the controllability and renaturation yield of the renaturation process. In addition, the renaturation and purification method of the invention optimizes the composition of the renaturation buffer. Compared with the prior art, the invention is more suitable for large-scale industrialization production, low requirement on equipment, simple operation, can obtain protein with high purity, good stability, can reduce the side effects of the final product in clinical practice, can improve the safety and effectiveness of drugs.本發明公開了一種重組人粒細胞刺激因子的復性及純化方法。具體而言,本發明藉由在復性前以緩衝液置換方式去除還原劑,提高了復性工藝的可控性和復性率。另外,本發明的復性純化方法還優化了復性緩衝液的組成。同現有技術相比較,本發明更加適合大規模工業化放大生產,對設備要求低,操作簡單,得到的蛋白純度高,穩定性好,可降低最終產品在臨床中的副作用,提高藥品的安全性和有效性。由於本案的圖為實驗數據,並非本案的代表圖。故本案無指定代表圖。
