A working casing pipe for minimally invasive surgery comprises a sheath (2), a silicone casing pipe (6), a multi-channel cap (9), and a multi-channel cushion (11). The multi-channel cap (9) and the multi-channel cushion (11) can be used for letting multiple instrument channels (8) pass through. Openings used for letting carbon dioxide channels (7) pass through are disposed on the multi-channel cap (9) and the multi-channel cushion (11). Multiple tracks (10) are disposed on the multi-channel cap to let the instrument channels (8) move left and right. Further, a gas injection hole (14) or a valve is disposed in the multi-channel cap (9) to fill gas into a gasbag (15) at the lower end of the silicone casing pipe (6). The multi-channel cushion (11) is corrugation-shaped, and the cross section may be V or U-shaped. The sheath (2) and the silicone casing pipe (6) are fitted together to fonn the middle part of the working casing pipe with both hard and soft materials. A penetrating channel (5) is located at the tail of the sheath (2) to let a penetrating knife pass through, and an angle is formed between the tail of the sheath (2) and the middle part of the sheath (2). The working casing pipe provides the possibility of direct penetration, enough support force and large operating space. It is flexible in operation and has functions of gas leakage preventing and coming-out preventing, thereby breaking the restriction of the existing working casing pipe on types and methods of the minimally invasive surgery and meeting requirements in complicated minimally invasive surgery.