ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM;CRAIG, ROGER KINGDON;CRAIG; ROGER KINGDON
发明人:
CRAIG, ROGER KINGDON,GROSVELD, FRANKLIN GERARDUS,JANSSENS, RICHARD WILHELM,DRABEK, DUBRAVKA
申请号：
CA2580336
公开号：
CA2580336C
申请日:
2005.07.22
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present invention relates to the manufacture of a diverse repertoire offunctional heavy chain-only antibodies that undergo affinity maturation, anduses thereof. The invention also relates to the manufacture and use of adiverse repertoire of class-specific heavy chain-only antibodies and to themanufacture and use of multivalent polypeptide complexes with antibody heavychain functionality, preferably antibody heavy chain binding functionality,constant region effector activity and, optionally, additional effectorfunctions. The present invention also relates to a method of generation offully functional heavy chain-only antibodies in transgenic mice in response toantigen challenge. In particular, the present invention relates to a methodfor the generation of human antigen-specific, high affinity, heavy chain-onlyantibodies of any class, or mixture of classes and the isolation andexpression of fully functional VH antigen-binding domains.
