The present invention provides an antiseptic and antibiotic composition comprising a Pseudomonas aeruginosa culture broth extract as an active ingredient. The present invention provides a composition for preventing or treating acne comprising an extract of Pseudomonas aeruginosa culture medium as an active ingredient. The composition containing the Pseudomonas aeruginosa culture solution extract of the present invention exhibits a broad antibacterial spectrum and a high antioxidant effect against various bacteria, and such a composition can be applied to a cosmetic composition such as cosmetics. Besides, it can be usefully used for antibacterial, sterilizing and antiseptic purposes in various fields such as foods, pharmaceuticals, agricultural chemicals and daily necessities. The present invention investigates specific active ingredients that cause the antibacterial effect of Pseudomonas aeruginosa culture extract and provides its structure. [Selection] Figure 1本発明は、緑膿菌培養液抽出物を有効成分として含む防腐用及び抗生組成物を提供する。本発明は、緑膿菌培養液抽出物を有効成分として含むにきびの予防または治療用組成物を提供する。本発明の緑膿菌培養液抽出物を含む組成物は、多様な菌に対して幅広い抗菌スペクトル及び高い抗酸化効果を示し、このような組成物は、化粧品のような化粧料組成物に適用が可能であり、それ以外にも、食品、医薬品、農薬及び生活用品などの多様な分野で抗菌、殺菌、防腐の目的として有用に利用可能である。本発明は、緑膿菌培養液抽出物の抗菌効果を引き起こす特定の有効成分を究明し、その構造を提供する。【選択図】図1