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PURIFICATION D'IMMUNOGLOBULINE
专利权人:
F. HOFFMANN-LA ROCHE AG
发明人:
申请号:
EP09796320.1
公开号:
EP2379108B1
申请日:
2009.12.18
申请国别(地区):
EP
年份:
2014
代理人:
摘要:
The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric, in aggregated, and in fragmented form to an anion exchange chromatography material under conditions whereby the immunoglobulin in monomeric form does not bind to the anion exchange material, and recovering the immunoglobulin in monomeric form in the flow-through from the anion exchange chromatography material, whereby the buffered aqueous solution has a pH value of from 8.0 to 8.5. In one embodiment the anion exchange chromatography material is a membrane anion exchange chromatography material.
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