The present disclosure relates to methods for producing dopaminergic cells and evaluating their functionality. When pluripotent human embryonic stem cells are cultured on plates coated with laminin 111 laminin 121 laminin 521 laminin 421 or laminin 511 in cell culture medium containing a GSK3 inhibitor and a TGF ß inhibitor as well as timely administered fibroblast growth factor desired neural cells are produced at far higher rates. Useful cell culture kits for producing such dopaminergic cells are also described herein as are methods of using such cells for stem cell therapy.
