A method of Separating a fraction of PRP from a whole Blood Sample into a Sampling tube which includes a Polymerizable substance separator.The separator is autosuspensible Polymerizable substance with whole Blood, has a First Average Molecular Weight.And has a Density between the average density of a fraction of the average density of PRP and PRP in a fraction of the substance, the separator includes an oligomer.A Photoinitiator and a Stabilizer.Understanding the Centrifuge Method: the whole Blood sample in the sampling tube which includes the Polymerizable substance separator for a period between one and thirty minutes between500 and 5000 rpm Making the centrifugal separator Polymerizable Substance flow between the fraction of PRP and PRP fraction not without substantial Activation of platelets in the fraction of PRP remains substantial, measurable Activation in terms of the ability of platelets in PRP fraction added to at least one of ristocetin and Collagen, tighten the separator substance,Through exposure to UV Energy over a period of not less than ten minutes, to form a strong BarrierTaking the Polymerizable substance separating the solid Barrier a Second Average Molecular Weight that is greater than the first Average Molecular Weight, being the strong BarrierAfter Exposure to the UV Energy,Stationary with respect to the sampling Tube and an intermediate position below the fraction of PRP and PRP on the fraction not that allows extraction of at least 95% of the FRAction of PRP in the sampling Tube and the extract fraction of PRP in the sampling Tube in a closed process that maintains the Sterility of the fraction of PRP,A Liquid Dispenser using a transfer Needle, which connects both the fraction of PRP and a First end of the dosing of Liquids,Understanding the different Liquid Dispenser, a Second end to the first end and the second end configured to distribute the fraction of PRP.Un método de separación de una fracción de PRP de una muestra de sangre total en u