EIGENBROT, Charles W.,JUNUTULA, Jagath Reddy,LOWMAN, Henry,RAAB, Helga E.,VANDLEN, Richard
申请号：
ES05811919
公开号：
ES2579805T3
申请日:
2005.09.22
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
A process for producing a modified cysteine engineered antibody comprising one or more free cysteine amino acids having a thiol reactivity value in the range of 0.6 to 1.0 and a sequence in the heavy chain selected from SEQ ID NOS: 10, 11, 13, 14, 31, May 34, 37, 38 and 42: NSLRCEDTAV (SEQ ID NO: 10) LVTVCSASTKGPS (SEQ ID NO: 11) LVTVSSCSTKGPS (SEQ ID NO: 13) LVTVSSACTKGPS (SEQ ID NO: 14) HEDPEVKFNWYVDGCEVHNAKTKPR (SEQ ID NO: 31) YKCKVCNKALP (SEQ ID NO: 34) KGFYPCDIAVE (SEQ ID NO: 37) PPVLDCDGSFF (SEQ ID NO: 38) GQGTLVTVSACSTKGPSVFPL (SEQ ID NO: 42) where cysteine at SEQ ID NOS: 10, 11, 13, 14, 31, 34, 37, 38 and 42 is the free amino acid cysteine or a sequence in the light chain selected from SEQ ID NOS: 17, 19, 20, 21, 22, 24, 25 and 27: SLSASCGDRVT (SEQ ID NO: 17) EIKRTCAAPSV (SEQ ID NO: 19) TCAAPCVFIFPP (SEQ ID NO: 20) FIFPPCDEQLK (SEQ ID NO: 21) DEQLKCGTASV (SEQ ID NO: 22) WKVDNCLQSGN (SEQ ID NO: 24) ALQSGCSQESV (SEQ ID NO: 25) GLS SPCTKSFN (SEQ ID NO: 27) where cysteine at SEQ ID NOS: 17, 19, 20, 21, 22, 24, 25 and 27 is the free amino acid cysteine wherein the process comprises replacing one or more amino acid residues of a parent antibody with the amino acid residue free cysteine wherein the parent antibody binds selectively to an antigen and antibody engineered cysteine selectively binds the same antigen as the parent antibody and wherein the antibody engineered cysteine is a human, chimeric or humanized antibody.Un proceso para producir un anticuerpo modificado por ingeniería genética con cisteína, que comprende uno o más aminoácidos cisteína libres que tienen un valor de reactividad del tiol en el intervalo de 0,6 a 1,0 y una secuencia en la cadena pesada seleccionada de las SEQ ID NOS: 10, 11, 13, 14, 31, 5 34, 37, 38 y 42: NSLRCEDTAV (SEQ ID NO:10) LVTVCSASTKGPS (SEQ ID NO:11) LVTVSSCSTKGPS (SEQ ID NO:13) LVTVSSACTKGPS (SEQ ID NO:14) HEDPEVKFNWYVDGCEVHNAKTKPR (SEQ ID NO:31) YKCKVCNKALP (SEQ ID NO:34) KGFYPCDIAVE (SEQ ID NO:37) PPVLDCDGSF
