Described herein are compositions and methods relating to the use of a combination of live lactobacilli bacteria, particularly live Lactobacillus acidophilus, live Lactobacillus casei, and live Lactobacillus rhamnosus, for the relief of undesirable gastrointestinal health problems such as irritable bowel syndrome (IBS), abdominal pain, abdominal discomfort, bloating, liquid stools and constipation. This combination of live lactobacilli bacteria can also be used for improving quality of life of a subject suffering from IBS, for the relief of IBS, and/or for the prevention and/or treatment of IBS.