A method of determining the location of the scleral spur, to allow measurements of the anterior chamber of the eye to facilitate diagnosis or treatment of the eye. The method utilizes ultrasonic biomicroscopy apparatus to image the anterior chamber and several millimeters of sclera and align indicators, or along the scleral-uveal interface and the corneal-aqueous interface. These indicators can be lines, dots or any shape or colors. The intersection of these indicators is used as an estimated location of the scleral spur for measuring anterior chamber angle, angle opening distance, or other aspects of the eye. The disclosure includes methods of locating the scleral spur, software for locating the scleral spur using computerized imaging apparatus, and systems including computerized imaging apparatus and software for locating the scleral spur.
