It discloses a composition of Chinese medicine for treating HPV papillomavirus, as well as its preparation method and formulation in this invention. The composition comprises momordica cochinchinensis 1-30, rhizoma bolbostemmae 1-30, leptochloa chinensis 1-30, lithospermum 1-20, xanthium 1-20, fructus psoraleae 1-18, cantharides 1-15 and cuttlebone 1-15 weight proportions. When the composition is used to treating HPV papillomavirus disease, especially condyloma acuminatum, it brings good effect, as well as less pain, no scar and little toxic to the patient, and it also has a low rate of relapse.本發明揭露一種用於治療人類乳突病毒(HPV)的中藥組合物、其製備方法及其製劑。該中藥組合物包括1-30重量份之木鱉子、1-30重量份之土貝母、1-30重量份之千金子、1-20重量份之紫草、1-20重量份之蒼耳子、1-18重量份之補骨脂、1-15重量份之斑蝥及1-15重量份之海螵蛸。在用於治療人類乳突病毒(HPV)疾病,尤其是尖銳濕疣時,具有讓患者疼痛感小而效果好、不易復發、不留瘢痕、毒副作用小的優點。