HERBAL FORMULATION FOR TREATMENT OF MASTITIS The present invention relates to a administered herbal formulation for treatment or prevention of mastitis in livestock. The herbal formulation comprises of an extract of Drynaria quercifolia wherein said herbal formulation can be administered orally, topically, intramammary route or parenterally. The herbal formulation comprises of a plant part of Drynaria quercifolia in an amount ranging from 50wt% to 90wt% and an additive in an amount ranging from 10 wt % to 50 wt %. The present invention also relates to a process for the preparation of extract of Drynaria quercifolia.