Disclosed are an insulin secretagogue peptide multi-dose hydro-injection pharmaceutical composition and use thereof. A long-term storage formulation of insulin secretagogue peptide can be obtained via the method of the present invention. The pharmaceutical composition of the present invention comprises: insulin secretagogue peptide, insulin secretagogue peptide analog and derivative； pharmaceutically acceptable osmotic pressure modifier (stabilizer)； pharmaceutically acceptable antiseptic； pharmaceutically acceptable dissolution promotor and pharmaceutically acceptable buffer solution. The pharmaceutical composition of the insulin secretagogue peptide is used in the preparation of drugs for treating diabetes and adiposis.
