A device and method for producing a portion of platelet rich plasma from a fluid sample obtained from a patient. The device comprises three intercommunicated portions, each portion having an internal chamber defined therein. The fluid sample is first brought into the base portion where it is separated into red blood cells and plasma. The plasma portion of the sample is then withdrawn into the second portion where it is further separated into platelet poor plasma disposed over platelet rich plasma. The portion of platelet poor plasma is then withdrawn from the second chamber, leaving only platelet rich plasma within the second chamber. The base portion also includes a membrane located on its distal end which helps maintain a vacuum within the device when the fluid sample is being initially inserted into base chamber.
