HENRY B. LOWMAN,LEONARD G. PRESTA,PAULA M. JARDIEU,JONH LOWE
申请号：
BR9810654
公开号：
BR9810654B8
申请日:
1998.06.30
申请国别(地区):
BR
年份:
2013
代理人:
摘要：
The present invention relates to a method for adjusting the affinity of a polypeptide to a target molecule by a combination of steps, including: (1) the identification of aspartyl residues which are prone to isomerization； (2) the substitution of alternative residues and screening the resulting mutants for affinity against the target molecule In a preferred embodiment, the method of subtituting residues is affinity maturation with phage display (AMPD). In a further preferred embodiment the polypeptide is an antibody and the target molecule is an antigen. In a further preferred embodiment, the antibody is anti-IgE and the target molecule is IgE. In another embodiment, the invention relates to an anti-IgE antibody having improved affinity to IgE.
