Disclosed is a method for modification of heat resistance of Newcastle disease virus and use thereof, according to which heat resistance of Newcastle disease virus can be distinctly improved through replacement of HN genes. The present disclosure takes advantage of reverse genetic manipulation technique. Modification of non-heat-resistant Newcastle disease virus is based on modification of transcription plasmid, which is achieved through replacing a HN gene of a non-heat-resistant Newcastle disease virus with a HN gene of a heat-resistant Newcastle disease virus. The modified transcription plasmid is used to transfect a host cell. A recombinant Newcastle disease virus is rescued and obtained. The recombinant Newcastle disease virus has a distinctly improved heat resistance. The present disclosure, for the first time, enormously improves the heat resistance characteristic of Newcastle disease virus through artificial recombinant, and successfully transforms the non-heat-resistant Newcastle disease vaccine LaSota strain into a heat-resistant Newcastle disease vaccines rT-HN strain. The method provided by the present disclosure can be widely used in modification of heat resistance of Newcastle disease virus strains, and has a broad application prospect in developing heat-resistant, safe, and efficient Newcastle disease vaccines.
