A METHOD FOR MASS PRODUCTION OF UNDARIOPSIS PETERSENIANA USING REGENERATION AND MATURATION INDUCTION OF FREE-LIVING GAMETOPHYTES OF UNDARIOPSIS PETERSENIANA
대한민국(관리부서:국립수산과학원);REPUBLIC OF KOREA(NATIONAL FISHERIES RESEARCH AND DEVELOPMENT INSTITUTE);목포대학교산학협력단;MOKPO NATIONAL UNIVERSITY INDUSTRY-ACADEMIA COOPERATION GROUP;REPUBLIC OF KOREA(NATIONAL FISHERIES RESEARCH ANDDEVELOPMENT INSTITUTE)
PURPOSE: A undaria peterseniana mass production method by inducing the regeneration and aging of undaria peterseniana free-living gametophytes is provided by securing the optimal condition for the regeneration and aging of the Undaria peterseniana free-living gametophytes. CONSTITUTION: Zoospores are released from an apothecium site of Undaria peterseniana. The released zoospores are cultured, and form gametophytes. Separate the gametophytes formed into female and male gametophytes. Culture the separated female and male gametophytes until the gametophytes reach the respective diameter of 3-10mm. Cut the cultured female and male gametophytes in the respective size of 50-400 micrometers. Culture the excised female and male gametophytes under the cell count of 3-25 cell/ind., the temperature condition of 5-20deg. C , the illumination of 5-40micromolm-2s-1, and the photoperiod of 10:14(Light:Dark)-14:10(L:D). Cross-fertilize the cultivated female and male gametophytes. Culture the gametophytes in a liquid culture medium of PESI(Provasoli`s enriched seawater media). Culture the female and male gametophytes until the gametophytes reach the diameter size of 5-6mm. Excise the female gametophytes in the size of 50-100 micrometers. Excise the male gametophytes in the size of 60-150 micrometers. Culture the female gametophytes under the cell count of 3-8 cell/ind., the temperature condition of 10-20 deg. C, the illumination of 15-30 micromolm-2s-1, and the photoperiod of 10:14(Light:Dark). Culture the male gametophytes under the cell count of 8-12 cell/ind., the temperature condition of 5-20 deg. C, the illumination of 15-25 micromolm-2s-1, and the photoperiod of 14:10(Light:Dark). [Reference numerals] (AA) Matured thallus of Undaria peterseniana； (BB) Rinsing the matured thallus, cut after washing, with sterilized seawater and ABM solution； (CC) Releasing zoospores at 10°；C, 20umolm^-2s^-1, and 10:14h(L:D)； (DD) 0.1ml zoospore solution； (EE) Separating the zoospores by dil
