LUBRIZOL GENETICS, INC.;THE UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, INC.
发明人:
COLLINS, GLENN B.,HILDEBRAND, DAVID F.,LAZZERI, PAUL A.,ADAMS, THOMAS R.,PARROTT, WAYNE A.,HARTWECK, LYNN M.
申请号：
US19870063342
公开号：
US5024944(A)
申请日:
1987.06.15
申请国别(地区):
美国
年份:
1991
代理人:
摘要：
A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.
