A sterilization assembly and methods of use for sterilizing various instruments or surfaces are described herein. The assembly may generally comprise an elongate housing defining a storage lumen and an opening, a plurality of cartridges aligned adjacent to one another within the storage lumen where each cartridge has a cleaning element, a biasing mechanism configured to bias the plurality of cartridges towards the opening of the storage lumen, and a release mechanism positioned along the elongate housing and adjacent to the opening. Actuation of the release mechanism may release at least one cartridge at the opening for ejection. The assembly may also comprise an actuator which oscillates the cleaning element or the at least one cartridge relative to the elongate housing. Furthermore, a processor in electrical communication with the actuator which is programmed to monitor operation of the actuator and/or positioning of the plurality of cartridges may also be incorporated.
