INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
发明人:
YUN, CHUL HO,윤철호,KIM, DONG HYUN,김동현,KANG, JI YEON,강지연,PARK, SUN HA,박선하,RYU, SANG HOON,류상훈
申请号：
KR1020120094935
公开号：
KR1020130055511A
申请日:
2012.08.29
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: A composition, a kit, and a method for preparing a 2-hydroxyl product or a 4-hydroxyl product from atorvastatin, are provided to cheaply, efficiently, and selectively produce a large amount of the 2-hydroxyl products or the 4-hydroxyl products, and to develop a novel drug using an atorvastatin metabolite.CONSTITUTION: A composition for preparing a 2-hydroxyl product or a 4-hydroxyl product from atorvastatin contains one or more enzymes selected from the group consisting of wild type CYP102A1, mutant CYP102A1, and a chimera of mutant CYP102A1. A method for preparing the 2-hydroxyl product or the 4-hydroxyl product from atorvastatin comprises a step of reacting the enzymes with atorvastatin. A kit for preparing the 2-hydroxyl product or the 4-hydroxyl product from atorvastatin contains the enzymes and an NADPH-generation system.COPYRIGHT KIPO 2013본 발명은 박테리아 시토크롬 P450을 이용한 아토르바스타틴으로부터 대사산물의 신규한 생산방법 및 이를 위한 조성물에 관한 것으로, 보다 상세하게는 박테리아 시토크롬 P450 BM3(CYP102A1), CYP102A1의 돌연변이체(mutant) 및 CYP102A1의 돌연변이체의 카메라(chimera)를 포함하는 아토르바스타틴으로부터 2-히드록실산물 또는 4-히드록실산물제조용 조성물, 키트 및 이의 생산방법에 관한 것이다.
