A single-use bag (32) and dispensing system (30) for storage of liquid or frozen media and for dispensing the media are disclosed. A single-use bag (32) is composed of materials that can withstand the temperatures required for dry heat sterilization and, further, includes specialized clamps (40, 140) that accommodate the materials and construction techniques necessary for constructing the single-use bag (32) for restriction and isolation of flow from the single-use bag (32). The single-use bag (32) includes an inner film (76) defining a bag interior (52) and an outer film (78) overlying the inner film (76), the inner film (76) and the outer film (78) being heat bonded together, the inner film (76) being composed of perfluoroalkoxy alkane (PFA) and the outer film (78) being composed of polytetrafluoroethylene (PTFE), the single-use bag (32) defining at least one exit port (62) on a bottom edge (48) thereof and at least one closeable exit passage (34) depending from the bottom edge (48) of the single-use bag (32), each of the at least one exit passage (34) being coupled to a respective one of the at least one exit port (62), the at least one exit passage (34) being in fluid communication with the bag interior (52).
