The present invention relates to a multi-domain vesicle comprising an immunoactive material, a production method for the multi-domain vesicle and an immunomodulatory composition comprising the multi-domain vesicle. According to one aspect of the present invention, the multi-domain vesicle comprises: at least two liposomes making contact and connected with each other； and a multi-domain vesicle outer wall surrounding the at least two liposomes. The multi-domain vesicle is formed from an oil phase and an aqueous phase, wherein: the oil phase comprises a first immunomodulatory material and a fluid oil； the oil phase forms a membrane of the liposomes, and the multi-domain vesicle outer wall； the aqueous phase comprises a second immunomodulatory material； the aqueous phase is an internal aqueous phase of the membrane of the liposomes, and an outer aqueous phase of the membrane of the liposomes； the first immunomodulatory material is a fat-soluble immunoactive material； the second immunomodulatory material is a water-soluble immunoactive material； and the fluid oil increases the structural stability of the at least two liposomes making contact and connected with each other.
