Disclosed herein are recombinant fusion polypeptides comprising one or more antigenic peptides from a cancer-associated protein (e. G., Fused to a PEST-containing peptide). The antigenic peptides may comprise one or more or all of the antigenic peptides comprising a repeated cancer mutation, an antigenic peptide comprising an irregularly mutated mutation, or an antigenic peptide fused to the ubiquitin protein. For example, provided herein are recombinant fusion polypeptides comprising two or more antigenic peptides (e.g., fused to a PEST-containing peptide), each antigenic peptide comprising a repeated cancer mutation, wherein at least two Antigenic peptides are fragments of the same cancer-associated protein. Also provided are nucleic acids encoding such fusion polypeptides, recombinant bacterial or listerial strains comprising such fusion polypeptides or such nucleic acids, and cell banks comprising such recombinant bacteria or Listeria strains. Also provided are methods of preparing such fusion polypeptides, such nucleic acids, and such recombinant bacterial or listerial strains. Also provided are immunogenic compositions, pharmaceutical compositions, and vaccines comprising such fusion polypeptides, such nucleic acids, or such recombinant bacterial or listerial strains. Also provided are methods of inducing anti-tumor-associated-antigen immune responses in a subject using such recombinant fusion polypeptides, nucleic acids, recombinant bacterial or listerial strains, immunogenic compositions, pharmaceutical compositions, or vaccines, There is provided a method of inducing an anti-cancer immune response, a method of treating a tumor or cancer in a subject, a method of preventing a tumor or cancer in a subject, and a method of protecting a subject against a tumor or cancer.본원에는 암-관련 단백질로부터의 하나 이상의 항원성 펩타이드 (예컨대, PEST-함유 펩타이드에 융합됨)를 포함하는 재조합 융합 폴리펩타이드가 제공된다. 항원성 펩타이드는 반복되는 암 돌연변이를 포함하는 항원성 펩타이드의 하나 이상 또는 전부, 불규칙변화성 돌연변이를 포함하는 항원성 펩타이드, 또는 유비퀴틴 단백질에 융합된 항원성 펩타이드를 포함
