A method for preparing an edge-rigidized artificial biological valve comprising: first soaking an artificial biological valve in a pretreated glutaraldehyde solution, wherein the artificial biological valve can be partly fixed with glutaraldehyde before, after or during contact with the pretreated glutaraldehyde solution and then closing the free aldehyde groups, carboxyl groups, amino groups, hydroxyl groups and carbonyl groups formed by the artificial biological valve pretreated with glutaraldehyde with a reducing agent and tannin extract. The edge rigidity of the artificial biological valve is enhanced, so that the long-term stability and durability of the artificial biological valve are improved.
