An illumination unit for endoscope is provided with: a light guiding layer, an illumination lens, light guides and a positioning member; and the positioning member is provided with: a barrel body on which arrangement grooves are formed, the barrel body being configured to perform inward positioning of the light guides in the radial direction by distal end sides of the light guides being arranged in the arrangement grooves; and a cylindrical member configured to perform outward positioning of the light guides in the radial direction by covering an outer circumference of the barrel body in an intersection direction to block the distal end sides of the light guides arranged in the arrangement grooves, between the cylindrical member and the barrel body in the intersection direction.