This disclosure provides a multifunctional intravascular tissue puncture needle and use thereof. The puncture needle comprises a split-shaped structure made of a memory metal and capable of opening and closing, the split-shaped structure is composed of a plurality of tapered petals; when each of tapered petals is closed, the split-shaped structure is a conical structure; when each of tapered petals is opened, the split-shaped structure is a cylindrical structure having a plurality of tapered notches on the wall, and the center of the cylindrical structure is an open cavity structure. The puncture needle can not only move well in the blood vessels, but also accurately pierce the blood vessel wall, and achieve effective sampling for biopsy.