ROO DE, Guy,VERSTEGEN, Ruud Martin,COUMANS, Rudy Gerardus Elisabeth
申请号：
ES15700117
公开号：
ES2687225T3
申请日:
2015.01.09
申请国别(地区):
ES
年份:
2018
代理人:
摘要：
The present invention relates to a method for purifying a mixture of cysteine-linked antibody-drug conjugates, wherein the amount of non-conjugated antibody is in the range of 0-40% by weight, using hydrophobic interaction chromatography (HIC). The mixture is loaded onto a preparative HIC column using a 0.2-1.5 M aqueous salt solution, in which non-conjugated antibody is collected in a flow-through fraction, followed by elution of a purified mixture of cysteine-linked antibody-drug conjugates using a 0-100 mM aqueous salt solution.
