Disclosed are anti-ageing compositions comprising banyan tree (Ficus Benghalensis), lotus (Nelumbo Nucifera), and clover (Trifolium Pratense) serum fractions. Further disclosed are methods of improving the appearance of aging skin comprises the step of applying a composition having an effective amount of banyan tree serum fraction, lotus serum fraction, and clover serum fraction to a sign of aging on a skin surface, wherein the composition is applied for a period of time sufficient to improve the appearance of the aging skin wherein claudin-1 and aquaporin-3 are upregulated and the moisture and barrier function of human epidermis is increased.
