Problem to be solved: to provide a screening method capable of quickly and conveniently selecting substances capable of increasing nitric oxide (no) production in vascular endothelial cells.A method of screening a substance that enhances nitric oxide (no) production of vascular endothelial cells, wherein in vitro, an nitric oxide indicator and an active oxygen indicator are added to a reaction solution containing a culture cell derived from a vascular endothelial cell, and a test substance is contacted to a culture cell The step of measuring the amount of nitric oxide and the amount of active oxygen, including the steps, the nitric oxide indicator and the active oxygen indicator, as well as measuring the amount of nitric oxide exceeds the reference value of nitric oxide, andA screening method that is considered to be a substance that enhances the nitric oxide production of vascular endothelial cells when the value of active oxygen is below the reference value of active oxygen.No selection【課題】血管内皮細胞の一酸化窒素(NO)産生を亢進する物質を、迅速かつ簡便に選抜できるスクリーニング方法の提供。【解決手段】血管内皮細胞の一酸化窒素(NO)産生を亢進する物質のスクリーニング方法であって、インビトロで、血管内皮細胞由来の培養細胞を含む反応液に一酸化窒素指示薬及び活性酸素指示薬を添加するステップと、培養細胞に被験物質を接触させるステップと、一酸化窒素指示薬及び活性酸素指示薬を指標として、一酸化窒素量及び活性酸素量を測定するステップと、を含み、一酸化窒素量の測定値が一酸化窒素の基準値を超え、かつ活性酸素量の測定値が活性酸素の基準値以下である場合、被験物質が血管内皮細胞の一酸化窒素産生を亢進する物質であると評価される、スクリーニング方法。【選択図】なし
