(주)마리아 바이오텍;LEE, Young Jae;박세영;PARK, Saeyoung;LIM, Jin Ho;이영재;MARIA BIOTECH;임진호
发明人:
LIM, Jin Ho,임진호,LEE, Young Jae,이영재,PARK, Saeyoung,박세영
申请号：
KRKR2011/005103
公开号：
WO2012/008733A3
申请日:
2011.07.12
申请国别(地区):
KR
年份:
2012
代理人:
摘要：
The present invention relates to stem cells derived from primary placenta tissue at 14 weeks or less of gestation, to a method for separation of same, to a method for proliferation of same, and to a cellular therapeutic agent containing the stem cells. More particularly, the present invention relates to stem cells derived from primary placenta tissue at 14 weeks or less of gestation, to a method for separation of same, to a method for proliferation of same, and to a cellular therapeutic agent containing the stem cells, wherein the stem cells: (a) exhibit positive immunological properties with respect to CD9, CD13, CD29, CD44, CD90, CD105 and HLA-class I, and negative immunological properties against CD14, CD19, CD34, CD38, CD45, CD127 and HLA-DR； (b) express genes of Oct4, Nanog, Tbn, Klf4, Activin and Rex1； (c) have the ability of differentiation into cells derived from an ectoderm, mesoderm, or endoderm； and are maintained for 30 generations or more in an undifferentiated state. In spite of being adult stem cells, adult stem cells derived from placenta tissue according to the present invention have excellent cell proliferation and advantageous differentiation ability compared to existing stem cells derived from placenta tissue, and thus can be efficiently used as a cellular therapeutic agent. In particular, the stem cells have the ability to differentiate into nerve cells, bone-forming cells, or pancreatic beta cells that secrete insulin, and are also efficient at treating bone defect diseases, nerve diseases, diabetes, etc.L'invention concerne des cellules couches issues de tissu de placenta primaire à 14 semaines ou moins de gestation, un procédé de séparation de ces cellules, un procédé permettant la prolifération de celles-ci et un agent thérapeutique cellulaire contenant ces cellules souches. Plus particulièrement, l'invention concerne des cellules souches issues de tissu de placenta primaire à 14 semaines ou moins de gestation, un procédé de séparation de ce
