A kit for lubricating dental floss comprises a flat base coupled to a panel defining a plurality of channels each containing a floss piece formed as an elongated loop having a bend and two free ends, lubrication zones, and compartments filled with lubricant, essentially formed of a flexible membrane, facing the respective lubrication zones. Each compartment includes dispenser orifices located near the bends, such that the vicinities of bends are lubricated upon a user's depressing on the membrane. An optional kit comprises a panel including cells each having dry and lubricant cavities, floss pieces each having a bend and two free ends, whereat the floss piece's middle portion is located in the lubricant cavity, the free ends are located in the dry cavity, and a flat cover coupled to the panel sealingly covering the cells. Preferred types of the floss and lubricant for the kits are also disclosed.