The present invention relates to a multiple-dose immunogenic composition for a multivalent combination vaccine, in which (i) the multivalent combination vaccine contains a whole cell pertussis (wP) vaccine or acellular pertussis (aP) vaccine, and a Sabin inactivated polio vaccine (sIPV)； and (ii) the composition contains 2-phenoxyethanol (2-PE) and formaldehyde (HCHO) as preservatives； and a method for preparing a multiple-dose immunogenic composition for a multivalent combination vaccine. It was confirmed that the combination of 2-phenoxyethanol and formaldehyde according to the present invention is an excellent preservative capable of maintaining antimicrobial ability and that it can be effectively used for providing a multiple-dose composition maintaining antimicrobial ability of combination vaccine, thus the composition of the present invention can be effectively used in the multiple-dose combination vaccine industry.
