Calculation method and calculation device for the abundance of phytoplankton of a specific species, and predictive detection method and predictive device for the occurrence of red tide by phytoplankton of a specific species
PROBLEM TO BE SOLVED: To easily calculate the abundance of a specific species of phytoplankton in a group of phytoplankton in which a plurality of types coexist. SOLUTION: The reference sample intensity ratio r 0 measured or calculated in the reference sample, the reference sample overall fluorescence intensity I 0 which is the intensity in substantially the entire wavelength band, and the presence of a specific species of phytoplankton that can be contained in the reference sample. on the basis of the quantity K 0 is calculated other types plankton intensity ratio r d, similarity with the reference sample is irradiated with excitation light to expected analytical sample with respect to the composition of the phytoplankton groups wavelength band a, in each of B, In addition to measuring the intensity of the fluorescence generated from the analysis sample, the intensity ratio r, which is the ratio of these two intensities, is calculated, and the overall fluorescence intensity I, which is the intensity of the fluorescence generated from the analysis sample in substantially the entire wavelength band, is measured. Based on the other species plankton intensity ratio rd, the intensity ratio r, and the total fluorescence intensity I, the abundance quantity K of the specific species of phytoplankton that can be contained in the analysis sample is calculated. [Selection diagram] Fig. 1【課題】複数の種類が混在する植物プランクトン群のうち特定種の植物プランクトンの存在量を簡便に算出する。【解決手段】基準試料において測定又は算出された基準試料強度比ｒ０と、略全波長帯域における強度である基準試料全体蛍光強度Ｉ０と、基準試料に含まれ得る特定種の植物プランクトンの存在数量Ｋ０とに基づいて他種プランクトン強度比ｒｄを算出し、植物プランクトン群の組成に関して基準試料との類似性が見込まれる分析試料に励起光を照射し、波長帯域Ａ，Ｂそれぞれにおいて、分析試料から生じる蛍光の強度を測定すると共に、これら２つの強度の比である強度比ｒを算出し、分析試料から生じる蛍光の、略全波長帯域における強度である全体蛍光強度Ｉを測定し、他種プランクトン強度比ｒｄと、強度比ｒと、全体蛍光強度Ｉに基づいて、分析試料に含まれ得る特定種の植物プランクトンの存在数量Ｋを算出する。【選択図】図１
