The present invention provides a new strain of bacillus atrophaeus MPL-01 (accession No: KCTC12329BP) which is gram-positive rhabdomeric, forms endogenous spores, and has antibacterial activity against chili pepper anthracnose. The MPL-01 strain and a culture medium thereof, according to the present invention, can be practically used as biological pesticides by effectively inhibiting chili pepper anthracnose.본 발명에 따라 신규의 바실러스 아트로페어스 균주인 MPL-01(기탁번호: KCTC12329BP)을 제공한다. 본 발명에 따른 MPL-01 균주와 그 배양액은 고추탄저병을 효과적으로 억제하여 생물 농약으로 유용하다.