PROBLEM TO BE SOLVED: To provide a low molecular weight proteoglycan having a skin-tightening effect, such as excellent effect of promoting collagen gel shrinkage and anti-glycation action.SOLUTION: According to the present invention, there is provided a low molecular weight proteoglycan. Proteoglycans are digested with proteolytic enzymes. The resulting low molecular weight proteoglycans have a protein content of less than 6% (weight ratio), and an uronic acid content of less than 35% (weight ratio). Here, a viscosity of pure water at 30°C was set to 1 and the relative viscosity of 0.5 w/v% aqueous solution is less than 2.0. There is maximum absorption at 275 nm in ultraviolet-visible absorption spectrum. The extinction coefficient per 1% is less than 0.1. In infrared absorption spectrum, there are absorption peaks at wave numbers 3423±10 cm-1, 1736±10 cm-1, 1638±10 cm-1, 1560±10 cm-1, 1378±10 cm-1, 1229±10 cm-1, and 1072±10 cm-1. In the proton nuclear magnetic resonance spectrum in heavy water measured at 70°C, there are signals at 2.02±0.05 ppm and 3.39±0.05 to 4.74±0.05 ppm.SELECTED DRAWING: Figure 7COPYRIGHT: (C)2017,JPO&INPIT【課題】優れたコラーゲンゲル収縮促進効果や抗糖化作用等の皮膚の引締め効果を有する低分子化プロテオグリカンの提供。【解決手段】プロテオグリカンをタンパク質分解酵素で消化し、タンパク質含量が6%(重量比)未満、ウロン酸含量が35%(重量比)未満で、30℃での純水の粘度1とし、0.5w/v%水溶液の相対粘度が2.0未満で、紫外可視吸収スペクトルで275nmに極大吸収があり1%当たりの吸光係数が0.1未満、赤外吸収スペクトルで波数3423±10cm-1、1736±10cm-1、1638±10cm-1、1560±10cm-1、1378±10cm-1、1229±10cm-1、及び1072±10cm-1に吸収ピークを有し、70℃で測定した重水中におけるプロトン核磁気共鳴スペクトルで2.02±0.05ppmと、3.39±0.05~4.74±0.05ppmにシグナルを有する低分子化プロテオグリカン。【選択図】図7
