Improved stem cell exosome-containing compositions are provided, along with methods for their preparation and use for regulating skin condition. The compositions provided contain isolated stem cell exosomes having increased levels of heat shock stress-response molecules. Uses of the exosome-containing compositions for regulating human skin include inducing increased skin integrity by cell renewal, enhancing water content or moisture of skin, reducing trans epidermal water loss, skin flaking, and scaling, improving skin thickness, enhancing skin tensile properties, reducing the appearance of dermal fine lines and wrinkles, improving skin texture, reducing skin pores size, enhancing skin smoothness, improving skin age spots, improving skin tone, and improving the appearance of scars and skin abrasions.
