The present invention provides: [1] a method for producing a retinal tissue characterized by comprising steps (1) to (3) i.e. (1) a first step of subjecting a pluripotent stem cell to floating culture in a serum free culture medium containing a Wnt signaling pathway inhibitor to form an aggregate of the pluripotent stem cell (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum free culture medium containing a basement membrane preparation and (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum containing culture medium； [2] a method for producing an optic cup like structure characterized by comprising a step of subjecting the aggregate containing a retinal tissue which has been cultured in the third step mentioned above to floating culture in a serum free culture medium or a serum containing culture medium each containing a substance capable of acting on the Sonic hedgehog signaling way and a substance capable of acting on the Wnt signaling pathway； [3] a method for producing a retinal pigment epithelial cell characterized by comprising a step of subjecting the aggregate containing a retinal tissue which has been cultured in the third step mentioned above to floating culture in a serum free culture medium or a serum containing culture medium each containing a substance capable of acting on the Wnt signaling pathway (wherein the serum free culture medium and the serum containing culture medium does not contain any substance capable of acting on the Sonic hedgehog signaling pathway)； and [4] a method for producing a retinal layer specific nerve cell characterized by comprising bringing a retinal progenitor cell contained in a retinal tissue derived from a primate pluripotent stem cell into contact with a Notch signaling pathway inhibitor.
