The present invention provides a biological tissue cutting device that can produce minute fragments of a biological tissue easily and efficiently. The biological tissue cutting device for producing tissue fragments by cutting a biological tissue includes: a first cutting unit configured to form a cut surface extending in a first direction by cutting the biological tissue on a stage and a second cutting unit configured to form a cut surface extending in a second direction and a cut surface extending in a third direction by cutting the biological tissue on the stage. The planar direction of the cut surface extending in the second direction is a direction crossing the cut surface extending in the first direction, and the planar direction of the cut surface extending in the third direction is a direction crossing the cut surface extending in the first direction and the cut surface extending in the second direction.
