The invention provides nucleic acids encoding acid α-glucosidase (GAA). In certain embodiments, nucleic acids have greater than about 86% sequence identity to a sequence selected from the group consisting of any of the sequences set forth as SEQ ID NOs:1-5. In certain embodiments, nucleic acids encoding acid α-glucosidase (GAA) contain less than 127 CpG dinucleotides. Expression cassettes, vectors, cells and cell lines and methods of using such nucleic acids encoding acid α-glucosidase (GAA) are also provided.本發明提供編碼酸性α葡萄糖苷酶(GAA)之核酸。在某些實施例中，核酸與選自由如SEQ ID NO:1-5所闡述之序列中之任一者組成之群之序列具有高於約86%的序列一致性。在某些實施例中，編碼酸性α葡萄糖苷酶(GAA)之核酸含有少於127個CpG二核苷酸。亦提供使用該等編碼酸性α葡萄糖苷酶(GAA)之核酸的表現盒、載體、細胞及細胞株及方法。
