#$%^&*AU2017203004A120171130. The method that pdf#####ABSTRACT operates disinfection system has vacuum chamber disinfecting apparatus, and room is connected to many disinfectants by valve closing state, is revealed. This method includes making instrument in the sterilizing battery pack of non-aseptic state, opens the room, makes to be fitted into chamber, room is closed, the first air is taken out from room, changes a large amount of liquid waters and is converted to steam, valve is opened, it introduces bactericidal agent and enters chamber, disinfectant is extracted out from chamber, is introduced the second volume of air and is entered the room, opens the room, packet is removed from the chamber, removes instruments sterility from battery pack. This method can also include with baseline humidity measurement and in the indoor pressure first pressure of chamber, reduce the pressure of the pressure in chamber to adjusting, kept for the pressure residence time adjusted, increase indoor pressure, and obtain the second moisture measurement from the chamber, benchmark moisture measurement and the second moisture measurement. 1/5 figure. The 36 24 40 30 32 44 2 processor fungicide reservoir lStcrage iiTimer 38 42 45 28 of supported V V of the pump load of 1 10 50 3,852 12 28 18 20 22 3 26 33 35 F