An alprostadil composition and injection and lyophilization formulation comprising the same the composition comprises alprostadil, oil for injection, phosphatidylcholine and phosphatidylinositol and is free of phosphatidylethanolamine and relative to 1 part by weight of alprostadil, the content of the oil for injection is 5000-30000 parts by weight, the content of the phosphatidylcholine is 1200-4000 parts by weight, and the content of the phosphatidylinositol is 12-48 parts by weight.Linvention a pour objet une composition dalprostadil, un liquide dinjection la contenant et sa formulation lyophilisée, la composition renfermant un alprostadil, une huile dinjection, une phosphatidylcholine et un phosphatidylinositol sans phosphatidyléthanol amine et comprenant 5000-30000 parties en poids dhuile dinjection, 1200-4000 parties en poids de phosphatidylcholine et 12-48 parties en poids de phosphatidylinositol.