This invention relates to synergistic microbicidal combinations of: 1) (A1) 2-methyl-4-isothiazolin-3-one with (B1): one or more compounds selected from the group consisting of anisic acid, decylene glycol, diethylene triamine pentacetic acid (DTPA), hydroxyethyl ethylenediamine triacetic acid (HEDTA), imminodisuceinate (IDS), maleic acid, methyl glycine diacetic acid (MDGA), phenoxypropanol, phytic acid, and propionic acid. 2) (A2) 1,2-benzisothiazolin-3-one with (B2): one or more compounds selected from the group consisting of anisic acid, capric acid, decytene glycol, diethylene triamine pentacetic acid (DTPA), etidronic acid, gluconic acid, hydroxyethyl ethylenediamine triacetic acid (HEDTA), imminodisuccinate (IDS), maleic acid, methyl glycine diacetic acid (MDGA), phenoxypropanol, phytic acid, and propionic acid. The examples show that abovementioned combinations have greater efficacy than would be expected from combinations of the individual components against one or more of following microorganisms: E. coli, C. albicans and A. niger.본 발명은 선택된 이소티아졸린-3-온 살미생물제와 제2 살미생물제 또는 제제 성분 또는 원료의 상승적 배합물에 관한 것이다. 이 배합물은 개별 성분들의 조합으로부터 예측되는 것보다 뛰어난 항미생물 활성을 지닌다. 상기 배합물은 (a) 메틸-4-이소티아졸린-3-온 또는 (b) 1,2-벤즈이소티아졸린-3-온과 하나 이상의 다른 여러 화합물의 혼합물을 포함한다.