A diaper, having urine detection functionalities, comprises a urine isolation layer, an absorbent core layer and an outer layer. A urine detection device is disposed in between the urine isolation layer and the absorbent core layer. The urine isolation layer has an open end embedded in a sleeve of the absorbent core layer. The urine detection device comprises a detection circuit and a test paper composite. The test paper composite includes: a trace element test paper, an antibiotic test paper and a urine routine test paper. Each test paper is made up of test strips for detecting different elements. Each test strip produces results according to a different color indicator scheme. The antibiotic test paper includes a specimen absorption pad, a specimen binding pad, a reaction membrane, and an absorbent pad. An antibiotic detection method is provided.
