HUANG, NING,RODRIQUEZ, RAYMOND L.,HAGIE, FRANK E.,STALKER, DAVID M.
申请号：
CA20032525493
公开号：
CA2525493(C)
申请日:
2003.04.11
申请国别(地区):
加拿大
年份:
2018
代理人:
摘要：
The present disclosure relates to a method of producing a recombinant human blood protein in Oryza plant seeds, comprising the steps of transforming an Oryza plant cell with a chimeric gene, growing an Oryza plant from the transformed Oryza plant cell for a time sufficient to produce seeds containing the human blood protein; and harvesting the seeds from the Oryza plant, wherein the human blood protein constitutes at least 3.0% of the total soluble protein in the harvested seeds. In the present disclosure, the chimeric gene comprises (i) a promoter from the gene of a maturation-specific monocot plant storage protein, wherein the promoter is operably linked to a first DNA molecule encoding a monocot plant seed-specific signal sequence capable of targeting a polypeptide linked thereto to a monocot plant seed endosperm cell, wherein the promoter operably linked to the first DNA molecule encoding the signal sequence has at least 95% sequence identity over the entire length of a sequence selected from the group co
